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Assay:

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Id: 30101
Copied From: 30100
 
GENERAL INFORMATION
 
Authors: Megan Davey
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: DCX_Probe_1_AS_DB
Gene Details
Gallus gallus Id: GG:14644
Entity Detected: DCX
Gene Name: doublecortin [Gallus gallus]
Entrez Id: 374242
Ensembl Id: ENSGALG00000007993
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: ISA Brown
Stage: HH23
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-02-08
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Neural tube lateral wall, dorsal root ganglion, hindbrain, neural folds, posterior limb mesenchyme, nasal placodes, telencephalon and midbrain.
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:446
  Name: dorsal root ganglion
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:817
  Name: hindbrain
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:1272
  Name: neural fold
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:788
  Name: hind limb mesenchyme
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note: Posterior region
 
Annotation ID: TMP:2343
  Name: wing mesenchyme
  Strength: Strong
  Pattern: Regional
  Direction:
  Note: Postetior region
 
Annotation ID: TMP:650
  Name: fronto-nasal process
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:2123
  Name: telencephalon
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:1178
  Name: midbrain
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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