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Id: 30083
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GENERAL INFORMATION
 
Authors: Megan Davey
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: GALNT9_Probe_2_AS_DB
Gene Details
Gallus gallus Id: GG:5975
Entity Detected: GALNT9
Gene Name: UDP-N-acetyl-alpha-D-galactosamine polypeptide N-acetylgalactosaminyltransferase 9 (GalNAc-T9) [Gallus gallus]
Entrez Id: 416796
Ensembl Id: ENSGALG00000002242
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: ISA Brown
Stage: HH22
Stage Qualifier: Unspecfied
Sex: Unspecified
Mutation: TALPID3
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-08-17
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Distal region of the limb, central limb mesenchyme and neural tube.
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:788
  Name: hind limb mesenchyme
  Strength: Strong
  Pattern: Regional
  Direction:
  Note: Central and distal region
 
Annotation ID: TMP:2343
  Name: wing mesenchyme
  Strength: Strong
  Pattern: Regional
  Direction:
  Note: Central and distal region
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Weak
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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