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Assay:

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Id: 30082
Copied From: 30081
 
GENERAL INFORMATION
 
Authors: Megan Davey
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: FZD10-1_Probe_1_AS_DB
Gene Details
Gallus gallus Id: GG:5989
Entity Detected: FZD10-1
Gene Name: frizzled homolog 10 (Drosophila) [Gallus gallus]
Entrez Id: 373885
Ensembl Id: ENSGALG00000002652
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: ISA Brown
Stage: HH22
Stage Qualifier: Unspecfied
Sex: Unspecified
Mutation: TALPID3
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-11-15
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Head mesenchyme, branchial arch IV and I, neural tube, intersomitic, distal limb mesenchyme and tail bud.
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:707
  Name: head mesenchyme
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:57
  Name: 4th branchial arch see 4th pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note: Intersomitic
 
Annotation ID: TMP:788
  Name: hind limb mesenchyme
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:2343
  Name: wing mesenchyme
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:2106
  Name: tail bud
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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