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Assay:

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Id: 30068
Copied From: 30067
 
GENERAL INFORMATION
 
Authors: Megan Davey
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 30067
Linked Data - Same Embryo:
 
PROBE
 
Probe: GCNT4_Probe_1_AS_DB
Gene Details
Gallus gallus Id: GG:23982
Entity Detected: GCNT4
Gene Name: glucosaminyl (N-acetyl) transferase 4 core 2 (beta-16-N-acetylglucosaminyltransferase) [Gallus gallus]
Entrez Id: 427207
Ensembl Id: ENSGALG00000014944
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: ISA Brown
Stage: HH23
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-07-06
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Somites in tail, distal posterior limb mesenchyme, head mesenchyme and branchial arch 4.
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:2115
  Name: tail somite
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:788
  Name: hind limb mesenchyme
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:707
  Name: head mesenchyme
  Strength: Not Detected
  Pattern: Homogeneous
  Direction:
  Note:
 
Annotation ID: TMP:57
  Name: 4th branchial arch see 4th pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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