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Id: 30052
Copied From: 30051
 
GENERAL INFORMATION
 
Authors: Megan Davey
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 30049, 30050, 30051
Linked Data - Same Embryo:
 
PROBE
 
Probe: FGF8_Probe_1_AS_DB
Gene Details
Gallus gallus Id: GG:17608
Entity Detected: FGF8
Gene Name: fibroblast growth factor 8 (androgen-induced) [Gallus gallus]
Entrez Id: 396313
Ensembl Id: ENSGALG00000007706
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Silkie
Stage: HH23
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2012-03-14
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Head ectoderm, Tail bud, nasal placode, AER (leg and wing), Somites and branchial arches
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:2106
  Name: tail bud
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:650
  Name: fronto-nasal process
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:161
  Name: apical ectodermal ridge
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:704
  Name: head ectoderm
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:23
  Name: 2nd branchial arch see 2nd pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:40
  Name: 3rd branchial arch see 3rd pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:57
  Name: 4th branchial arch see 4th pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:817
  Name: hindbrain
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:1178
  Name: midbrain
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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