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Assay:

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Id: 20063
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GENERAL INFORMATION
 
Authors: monique
Lab: University of Bath
Standard Protocol Id: Bath1
Lab Book Reference: lab book page 8 13_2_2009
Confidence: Moderate
Status: Public
Notes For Interpretation: heart removed, embryo scanned upside down
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20064|20065
Linked Data - Same Embryo:
 
PROBE
 
Probe: MME_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:19847
Entity Detected: MME
Gene Name: membrane metallo-endopeptidase [Gallus gallus]
Entrez Id: 425031
Ensembl Id: ENSGALG00000010331
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-02-13
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchyme
Not Detected In:
Possibly Detected In: somites
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: THE CHICK EMBRYO APPEARS AS A NATURAL MODEL FOR RESEARCH IN BETA-AMYLOID PRECURSOR PROTEIN PROCESSING J. A. CARRODEGUAS,a1,2 A. RODOLOSSE,a1,2 M. V. GARZA,a2 A. SANZ-CLEMENTE,a R. PÉREZ-PÉ,a2 A. M. LACOSTA,a L. DOMÍNGUEZ,a I. MONLEÓN,a R. SÁNCHE
 
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