| Id: |
30000 |
| Copied From: |
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| |
| GENERAL INFORMATION |
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| Authors: |
Frances Wong |
| Lab: |
Roslin Institute |
| Standard Protocol Id: |
Roslin |
| Lab Book Reference: |
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| Confidence: |
High |
| Status: |
Public |
| Notes For Interpretation: |
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| Linked Data - Replicated Experiment: |
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| Linked Data - Same Experiment: |
30001, 30002 |
| Linked Data - Same Embryo: |
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| PROBE |
| |
| Probe: |
CHRD_Probe_1_AS_DB |
| Gene Details |
| Gallus gallus Id: |
GG:20044 |
| Entity Detected: |
CHRD |
| Gene Name: |
chordin [Gallus gallus] |
| Entrez Id: |
395828 |
| Ensembl Id: |
n/a |
| |
| Chemistry: |
RNA |
| Label: |
Digoxigenin |
| |
| SPECIMEN |
| |
| Organism: |
Gallus gallus |
| Breed: |
White Leghorn |
| Stage: |
HH15 |
| Stage Qualifier: |
Exact |
| Sex: |
Unspecified |
| Mutation: |
None (Wild-Type) |
| Mounting: |
Wholemount |
| Fixative: |
4% PFA |
| Embedding Reagent: |
|
| |
| HYBRIDIZATION PROCEDURE |
| |
| Hybridization Date: |
2010-07-12 |
| Secondary: |
Anti-Digoxigenin Ab |
| Tertiary: |
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| Quaternary: |
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| Final Label: |
Alkaline Phosphatase |
| Detected Using: |
NBT+BCIP |
| Development Time: |
15 |
| Time Unit: |
Minutes |
| |
| IMAGING PROCEDURE |
| |
| Image Technique: |
OPT |
| Clearing Agent: |
BABB |
| |
| IMAGE FILES |
| |
| Local Zip File or Comment: |
|
| Number of Image Sets: |
1 |
| Image Folder: |
bioptonics |
| Image Quality: |
High |
| |
| EXPRESSION PATTERN DESCRIPTION |
| |
| Detected In: |
distal end of notochord |
| Not Detected In: |
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| Possibly Detected In: |
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| ANNOTATIONS |
| |
All site content,
except where otherwise noted,
