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Assay:

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Id: 40342
Copied From: 40111
 
GENERAL INFORMATION
 
Authors: Claire Anderson
Lab: University College London
Standard Protocol Id: ucl
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo: 40111
 
PROBE
 
Probe: MSX1_Probe_1_AS_CS
Gene Details
Gallus gallus Id: GG:15601
Entity Detected: MSX1
Gene Name: msh homeobox 1 [Gallus gallus]
Entrez Id: 396484
Ensembl Id: ENSGALG00000015013
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH11
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount subsequently Sectioned
Fixative: 4% PFA
Embedding Reagent: Paraffin
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2010-11-15
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 3
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1776
  Name: roof plate
  Strength: Strong
  Pattern: Regional
  Direction:
  Note: dorsal neural tube
 
Annotation ID: TMP:1995
  Name: splanchnic lateral plate mesoderm
  Strength: Weak
  Pattern: Regional
  Direction:
  Note: lateral
 
 
MISCELLANEOUS
 
References: 7553857
 
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