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Assay:

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Id: 40317
Copied From: 40162
 
GENERAL INFORMATION
 
Authors: Claire Anderson
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 40318
Linked Data - Same Embryo:
 
PROBE
 
Probe: PCSK6_Probe_1_AS_CS
Gene Details
Gallus gallus Id: GG:3736
Entity Detected: PCSK6
Gene Name: proprotein convertase subtilisin/kexin type 6 [Gallus gallus]
Entrez Id: 395454
Ensembl Id: ENSGALG00000007238
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH14
Stage Qualifier: Late (+)
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-09-07
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 12
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Strong
  Pattern: Regional
  Direction:
  Note: newly formed somites
 
Annotation ID: TMP:736
  Name: Hensens node
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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