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Id: 40278
Copied From: 40277
 
GENERAL INFORMATION
 
Authors: Anna Abraham / Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 1-1-2009
Confidence: High
Status: Public
Notes For Interpretation: protocol: Bath 1.1
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: LMO1_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:16081
Entity Detected: LMO1
Gene Name: LIM domain only 1 (rhombotin 1) [Gallus gallus]
Entrez Id: 423059
Ensembl Id: ENSGALG00000005967
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH21
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-01-01
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References: Bangs et al. 2010, Welten et al. 2011
 
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