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Assay:

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Id: 20054
Copied From:
 
GENERAL INFORMATION
 
Authors: monique
Lab: University of Bath
Standard Protocol Id: Bath1
Lab Book Reference: lab book page 6/ 05/02/09
Confidence: Moderate
Status: Public
Notes For Interpretation: heart removed, embryo scanned upside down
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20054|20056
Linked Data - Same Embryo:
 
PROBE
 
Probe: DOC2BL-1_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:7695
Entity Detected: DOC2BL-1
Gene Name: double C2-like domains beta-like [Gallus gallus]
Entrez Id: 772026
Ensembl Id: ENSGALG00000004917
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-02-05
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchyme|neural tube
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: DOC2A and DOC2B are sensors for neuronal activity with unique calcium-dependent and kinetic properties Alexander J. A. Groffen,*,1 Reut Friedrich,_,1 Elisabeth C. Brian,* Uri Ashery_ and Matthijs Verhage* Journal of Neurochemistry, 2006, 97, 818–833
 
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