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Id: 40270
Copied From: 40269
 
GENERAL INFORMATION
 
Authors: Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 9 May 2011
Confidence: Moderate
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: TMTC2_Probe_2_AS_CS
Gene Details
Gallus gallus Id: GG:591
Entity Detected: TMTC2
Gene Name: transmembrane and tetratricopeptide repeat containing 2 [Gallus gallus]
Entrez Id: 417880
Ensembl Id: ENSGALG00000010975
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-05-09
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: prototype_bath
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Weak
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2123
  Name: telencephalon
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Weak
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Weak
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:23
  Name: 2nd branchial arch see 2nd pharyngeal arch)
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note: located more proximally than in 1st branchial arch
 
Annotation ID: TMP:40
  Name: 3rd branchial arch see 3rd pharyngeal arch)
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note: see 2nd branchial arch
 
Annotation ID: TMP:1669
  Name: rhombencephalon
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References: Promoter is bound by HIF-1α and HIF-2α (Hypoxia Induced Factors) in MCF-7 and Tmtc2 is downregulated upon silencing of HIF-1α and HIF-2α. Mole 2009 J Biol Chem. PMID: 19386601 TMTC2 expression upregulated in Neurogenin-1 over-expressing human neural
 
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