Submission Interface

Assay:

Back
Id: 40268
Copied From:
 
GENERAL INFORMATION
 
Authors: Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 9 May 2011
Confidence: Moderate
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: PKIG_Probe_1_AS_CS
Gene Details
Gallus gallus Id: GG:10009
Entity Detected: PKIG
Gene Name: protein kinase (cAMP-dependent catalytic) inhibitor gamma [Gallus gallus]
Entrez Id: 419195
Ensembl Id: ENSGALG00000004177
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-05-09
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: prototype_bath
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:715
  Name: heart
  Strength: Weak
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References: PKIγ terminates PKA induced early gene expression. Chen et al. 2005 J Biol Chem PMID: 15557275 PKIγ expression is downregulated in human mesenchymal stromal cells when induced towards osteoblastic differentiation with BMP2. Zhao et al. 2006 Int J Bioch
 
Back