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Id: 40267
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GENERAL INFORMATION
 
Authors: Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 9 May 2011
Confidence: Moderate
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: VTN-1_Probe_1_AS_CS
Gene Details
Gallus gallus Id: GG:7608
Entity Detected: VTN-1
Gene Name: vitronectin [Gallus gallus]
Entrez Id: 395935
Ensembl Id: ENSGALG00000003589
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Early (-)
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-05-09
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: prototype_bath
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Moderate
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Strong
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Strong
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:715
  Name: heart
  Strength: Weak
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Weak
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:23
  Name: 2nd branchial arch see 2nd pharyngeal arch)
  Strength: Moderate
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:40
  Name: 3rd branchial arch see 3rd pharyngeal arch)
  Strength: Moderate
  Pattern: Graded
  Direction: Unknown
  Note:
 
Annotation ID: TMP:57
  Name: 4th branchial arch see 4th pharyngeal arch)
  Strength: Moderate
  Pattern: Graded
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References: Preissner, 1991 Annu Rev Cell Biol. PMID: 1725600.Vitronectin is cleaved in the embryonic chick notochord, floor plate and ventral neural tube into 2 shorter forms. Shh binds to all three forms (full and 2 short forms), but preferentially to a 45kDa prote
 
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