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Id: 40264
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GENERAL INFORMATION
 
Authors: Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 9 May 2011
Confidence: Low
Status: Public
Notes For Interpretation: low signal:noise, high background, trapping
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: NXPH1_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:8232
Entity Detected: NXPH1
Gene Name: neurexophilin 1 [Gallus gallus]
Entrez Id: 420582
Ensembl Id: ENSGALG00000010711
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-05-09
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: prototype_bath
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:1669
  Name: rhombencephalon
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:1118
  Name: mesencephalon
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Weak
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Weak
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2123
  Name: telencephalon
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References: secreted glycoproteins resembling neuropeptides. Binds to α neurexins (ubiquitous receptor-like proteins expressed by neurons). Missler et al. 1998 J Boil Chem PMID: 9856994. Expressed in subpopulations of neurons in adult rat brain (Patrenko 1996 J Neu
 
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