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Id: 40236
Copied From: 40235
 
GENERAL INFORMATION
 
Authors: Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 31-3-2011
Confidence: Moderate
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: MCF2L_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:2463
Entity Detected: MCF2L
Gene Name: MCF.2 cell line derived transforming sequence-like [Gallus gallus]
Entrez Id: 418748
Ensembl Id: ENSGALG00000016834
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH28
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-04-04
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 20
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:23
  Name: 2nd branchial arch see 2nd pharyngeal arch)
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Strong
  Pattern: Graded
  Direction:
  Note: looks like it is expressed in Cartilage
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Strong
  Pattern: Graded
  Direction:
  Note: looks like it is expressed in Cartilage
 
Annotation ID: TMP:302
  Name: central nervous system
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:1669
  Name: rhombencephalon
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2123
  Name: telencephalon
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:557
  Name: external naris
  Strength: Strong
  Pattern: Graded
  Direction:
  Note: strong expression found in nasal pits - couldn't find this term so entered what I thought was most likely the nasal pits
 
 
MISCELLANEOUS
 
References: High expression in adult rat heart and brain, particularly within neurons and alpha-tanycytes of the median eminence/hypothalamus. Horii et al 1994 EMBO J. PMID: 7957046.
 
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