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Id: 40232
Copied From: 40231
 
GENERAL INFORMATION
 
Authors: Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 30-3-2011
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 40231
Linked Data - Same Embryo:
 
PROBE
 
Probe: LMO7_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:2618
Entity Detected: LMO7
Gene Name: LIM domain 7 [Gallus gallus]
Entrez Id: 418815
Ensembl Id: ENSGALG00000016920
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-04-04
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 20
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:23
  Name: 2nd branchial arch see 2nd pharyngeal arch)
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2123
  Name: telencephalon
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References: Holaska et al 2006 Hum Mol Genet. PMID: 17067998. MGI:1353586. Expressed in ZF, chick and mouse CNS structures, vesicles, somites, gut, heart, limb/fin buds (but not mouse limbs). Knockout in ZF results in morphological and functional heart defects. Ott
 
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