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Assay:

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Id: 40209
Copied From:
 
GENERAL INFORMATION
 
Authors: Monique
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference: 15 March 2011
Confidence: Moderate
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: LMO7_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:2618
Entity Detected: LMO7
Gene Name: LIM domain 7 [Gallus gallus]
Entrez Id: 418815
Ensembl Id: ENSGALG00000016920
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH21
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-03-17
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 20
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:715
  Name: heart
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:3
  Name: 1st branchial arch see 1st pharyngeal arch)
  Strength: Strong
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:24
  Name: 2nd pharyngeal arch
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Moderate
  Pattern: Graded
  Direction:
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Weak
  Pattern: Graded
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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