Submission Interface

Assay:

Back
Id: 20041
Copied From:
 
GENERAL INFORMATION
 
Authors: monique
Lab: University of Bath
Standard Protocol Id: Bath1
Lab Book Reference:
Confidence: Moderate
Status: Public
Notes For Interpretation: heart removed, embryo scanned upside down
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20039|20040
Linked Data - Same Embryo:
 
PROBE
 
Probe: COLEC12_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:9195
Entity Detected: COLEC12
Gene Name: collectin sub-family member 12 [Gallus gallus]
Entrez Id: 421061
Ensembl Id: ENSGALG00000014910
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-01-19
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 35
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchyme|notochord|branchial arches|somites
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: Collectins Players of the innate immune system J. Koenraad van de Wetering, Lambert M. G. van Golde and Joseph J. Batenburg. Eur. J. Biochem. 271,1229–1249 (2004) _ FEBS 2004
 
Back