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Assay:

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Id: 20036
Copied From:
 
GENERAL INFORMATION
 
Authors: monique
Lab: University of Bath
Standard Protocol Id: Bath1
Lab Book Reference: 19/01/2009
Confidence: Moderate
Status: Public
Notes For Interpretation: heart removed,embryo scanned upside down
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo: 20037|20038
 
PROBE
 
Probe: TCERG1L_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:17843
Entity Detected: TCERG1L
Gene Name: transcription elongation regulator 1-like [Gallus gallus]
Entrez Id: 423969
Ensembl Id: ENSGALG00000010470
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-01-19
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 35
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchyme|neural tube
Not Detected In: anterior limb mesenchyme
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: Molecular mapping of developing dorsal horn-enriched genes by microarray and dorsal/ventral subtractive screening Mei-Zhang Li, Jin-Shan Wang, Dao-Jun Jiang, Chuan-Xi Xiang, Feng-Yang Wang, Kai-Hua Zhang, Philip R. Williams, Zhou-Feng Chen Developmen
 
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