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Assay:

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Id: 20034
Copied From:
 
GENERAL INFORMATION
 
Authors: Anna Abraham / Gordana / Monique
Lab: University of Bath
Standard Protocol Id: Bath1.1
Lab Book Reference: lab book page 28 // 28-10-2009
Confidence: Moderate
Status: Public
Notes For Interpretation: embryo scanned upside down
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20033|20035
Linked Data - Same Embryo:
 
PROBE
 
Probe: LMO1_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:16081
Entity Detected: LMO1
Gene Name: LIM domain only 1 (rhombotin 1) [Gallus gallus]
Entrez Id: 423059
Ensembl Id: ENSGALG00000005967
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH27
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-10-28
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using:
Development Time: 40
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchyme (weak)|neural tube (weak)
Not Detected In: anterior limb mesenchyme
Possibly Detected In: rhombencephalon
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: Zeng, C., Justice, N.J., Abdelilah, S., Chan, Y.N., Jan, L.Y., Jan, Y.N., 1998. The Drosophila LIM-only gene, dLMO, is mutated in Beadex alleles and might represent an evolutionarily conserved function in appendage development. Proc. Natl. Acad. Sci. USA
 
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