| Id: |
20291 |
| Copied From: |
20290 |
| |
| GENERAL INFORMATION |
| |
| Authors: |
Gordana/Hannah |
| Lab: |
University of Bath |
| Standard Protocol Id: |
Bath1.1 |
| Lab Book Reference: |
|
| Confidence: |
High |
| Status: |
Public |
| Notes For Interpretation: |
|
| Linked Data - Replicated Experiment: |
|
| Linked Data - Same Experiment: |
20290|20292|20293|20294 |
| Linked Data - Same Embryo: |
|
| |
| PROBE |
| |
| Probe: |
SHH_Probe_1_AS_CT |
| Gene Details |
| Gallus gallus Id: |
GG:8012 |
| Entity Detected: |
SHH |
| Gene Name: |
sonic hedgehog homolog (Drosophila) [Gallus gallus] |
| Entrez Id: |
395615 |
| Ensembl Id: |
ENSGALG00000006379 |
| |
| Chemistry: |
RNA |
| Label: |
Digoxigenin |
| |
| SPECIMEN |
| |
| Organism: |
Gallus gallus |
| Breed: |
White Leghorn |
| Stage: |
HH27 |
| Stage Qualifier: |
Exact |
| Sex: |
Unspecified |
| Mutation: |
None (Wild-Type) |
| Mounting: |
Wholemount |
| Fixative: |
4% PFA |
| Embedding Reagent: |
|
| |
| HYBRIDIZATION PROCEDURE |
| |
| Hybridization Date: |
2010-08-15 |
| Secondary: |
Anti-Digoxigenin Ab |
| Tertiary: |
|
| Quaternary: |
|
| Final Label: |
|
| Detected Using: |
NBT/BCIP concentration 2x lower than in Bath1 |
| Development Time: |
30 |
| Time Unit: |
Minutes |
| |
| IMAGING PROCEDURE |
| |
| Image Technique: |
OPT |
| Clearing Agent: |
BABB |
| |
| IMAGE FILES |
| |
| Local Zip File or Comment: |
|
| Number of Image Sets: |
1 |
| Image Folder: |
bioptonics |
| Image Quality: |
Unknown |
| |
| EXPRESSION PATTERN DESCRIPTION |
| |
| Detected In: |
posterior dorsal tip of wing bud|distal region of notochord floor plate |
| Not Detected In: |
|
| Possibly Detected In: |
eye |
| |
| ANNOTATIONS |
| |
All site content,
except where otherwise noted,
