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Assay:

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Id: 20027
Copied From:
 
GENERAL INFORMATION
 
Authors: monique
Lab: University of Bath
Standard Protocol Id: Bath1
Lab Book Reference: lab book page 4 //19/01/09
Confidence: Moderate
Status: Public
Notes For Interpretation: heart removed, embryo scanned upside down
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20028|20029
Linked Data - Same Embryo:
 
PROBE
 
Probe: TLE4_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:24148
Entity Detected: TLE4
Gene Name: transducin-like enhancer of split 4 (E(sp1) homolog Drosophila) [Gallus gallus]
Entrez Id: 374080
Ensembl Id: ENSGALG00000015184
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-01-19
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 35
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchyme|branchial arches
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: Combinatorial expression patterns of individual TLE proteins during cell determination and differentiation suggest non-redundant functions for mammalian homologs of Drosophila Groucho. Yao, J., Liu, Y. Husain, J. Lo, R., Palaparti, A., Henderson, J. and
 
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