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Assay:

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Id: 20026
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GENERAL INFORMATION
 
Authors: monique
Lab: University of Bath
Standard Protocol Id: Bath1
Lab Book Reference: lab book page 4 // 19/01/09
Confidence: Moderate
Status: Public
Notes For Interpretation: heart and head removed
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20024|20025
Linked Data - Same Embryo:
 
PROBE
 
Probe: LOC422563_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:15035
Entity Detected: LOC422563
Gene Name: similar to scrapie responsive protein 1 [Gallus gallus]
Entrez Id: 422563
Ensembl Id: ENSGALG00000010755
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-01-19
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 35
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchyme|branchial arches|notochord|neural tube
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: Mouse Scrapie Responsive Gene 1 (Scrg1): Genomic Organization, Physical Linkage to Sap30, Genetic Mapping on Chromosome 8,and Expression in Neuronal Primary Cell Cultures Michel Dron, Xavier Tartare, Fre´de´ ric Guillo, Ste´phane Haik,* Gilles Barbin
 
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