Submission Interface

Assay:

Back
Id: 20266
Copied From:
 
GENERAL INFORMATION
 
Authors: Cherry
Lab: University of Bath
Standard Protocol Id: Bath1.1
Lab Book Reference: 01-0_2010
Confidence: High
Status: Public
Notes For Interpretation: No brightfield image
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20267
Linked Data - Same Embryo:
 
PROBE
 
Probe: HOXD11_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:18183
Entity Detected: HOXD11
Gene Name: homeobox D11 [Gallus gallus]
Entrez Id: 395328
Ensembl Id: ENSGALG00000009273
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH24
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2010-06-01
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label:
Detected Using: NBT/BCIP concentration 2x lower than in Bath1
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: posterior limb mesenchymal| tail
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References:
 
Back