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Assay:

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Id: 41519
Copied From: 41518
 
GENERAL INFORMATION
 
Authors: Claire Anderson
Lab: University College London
Standard Protocol Id: ucl
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: TSPAN6_Probe_1_AS_CS
Gene Details
Gallus gallus Id: GG:14516
Entity Detected: TSPAN6
Gene Name: tetraspanin 6 [Gallus gallus]
Entrez Id: 422262
Ensembl Id: ENSGALG00000006808
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH10
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount subsequently Sectioned
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-07-19
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label:
Detected Using: NBT+BCIP
Development Time: 10
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1304
  Name: notochord
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:477
  Name: ectoderm
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1995
  Name: splanchnic lateral plate mesoderm
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:142
  Name: anterior intestinal portal
  Strength: Moderate
  Pattern: Regional
  Direction: Up
  Note: endoderm
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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