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Assay:

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Id: 41459
Copied From: 41458
 
GENERAL INFORMATION
 
Authors: Claire Anderson
Lab: University College London
Standard Protocol Id: ucl
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: TBX5_Probe_2_AS_CS
Gene Details
Gallus gallus Id: GG:6389
Entity Detected: TBX5
Gene Name: T-box 5 [Gallus gallus]
Entrez Id: 373988
Ensembl Id: ENSGALG00000008253
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH12
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2013-10-16
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 10
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:715
  Name: heart
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1362
  Name: optic vesicle
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:909
  Name: intermediate mesoderm
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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