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Assay:

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Id: 41337
Copied From: 41336
 
GENERAL INFORMATION
 
Authors: Claire Anderson
Lab: University College London
Standard Protocol Id: ucl
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: ID2_Probe_1_AS_CS
Gene Details
Gallus gallus Id: GG:13997
Entity Detected: ID2
Gene Name: inhibitor of DNA binding 2 dominant negative helix-loop-helix protein [Gallus gallus]
Entrez Id: 395852
Ensembl Id: ENSGALG00000016403
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH08
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount subsequently Sectioned
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2015-05-05
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 6
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:506
  Name: endoderm
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note: Anterior intestinal portal
 
Annotation ID: TMP:1995
  Name: splanchnic lateral plate mesoderm
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1277
  Name: neural plate
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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