| Id: |
20157 |
| Copied From: |
20156 |
| |
| GENERAL INFORMATION |
| |
| Authors: |
Gordana |
| Lab: |
University of Bath |
| Standard Protocol Id: |
Bath1.1 |
| Lab Book Reference: |
06-07_2009 |
| Confidence: |
High |
| Status: |
Public |
| Notes For Interpretation: |
|
| Linked Data - Replicated Experiment: |
|
| Linked Data - Same Experiment: |
20156|20158 |
| Linked Data - Same Embryo: |
|
| |
| PROBE |
| |
| Probe: |
E2F4_Probe_1_AS_CT |
| Gene Details |
| Gallus gallus Id: |
GG:3932 |
| Entity Detected: |
E2F4 |
| Gene Name: |
E2F transcription factor 4 p107/p130-binding [Gallus gallus] |
| Entrez Id: |
769430 |
| Ensembl Id: |
ENSGALG00000001722 |
| |
| Chemistry: |
RNA |
| Label: |
Digoxigenin |
| |
| SPECIMEN |
| |
| Organism: |
Gallus gallus |
| Breed: |
White Leghorn |
| Stage: |
HH21 |
| Stage Qualifier: |
Exact |
| Sex: |
Unspecified |
| Mutation: |
None (Wild-Type) |
| Mounting: |
Wholemount |
| Fixative: |
4% PFA |
| Embedding Reagent: |
|
| |
| HYBRIDIZATION PROCEDURE |
| |
| Hybridization Date: |
2009-07-06 |
| Secondary: |
Anti-Digoxigenin Ab |
| Tertiary: |
|
| Quaternary: |
|
| Final Label: |
|
| Detected Using: |
NBT/BCIP concentration 2x lower than in Bath1 |
| Development Time: |
35 |
| Time Unit: |
Minutes |
| |
| IMAGING PROCEDURE |
| |
| Image Technique: |
OPT |
| Clearing Agent: |
BABB |
| |
| IMAGE FILES |
| |
| Local Zip File or Comment: |
|
| Number of Image Sets: |
1 |
| Image Folder: |
bioptonics |
| Image Quality: |
Unknown |
| |
| EXPRESSION PATTERN DESCRIPTION |
| |
| Detected In: |
neural tube | first pharyngeal arch: mandibular process | first pharyngeal arch: maxillary process | limb mesenchyme
|
| Not Detected In: |
|
| Possibly Detected In: |
otic vesicle
|
| |
| ANNOTATIONS |
| |
All site content,
except where otherwise noted,
