Submission Interface

Assay:

Back
Id: 30246
Copied From:
 
GENERAL INFORMATION
 
Authors: Andrew Bain
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: DLX5-1_Probe_1_AS_DB
Gene Details
Gallus gallus Id: GG:8219
Entity Detected: DLX5-1
Gene Name: distal-less homeobox 5 [Gallus gallus]
Entrez Id: 373969
Ensembl Id: ENSGALG00000009129
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH20
Stage Qualifier: Exact
Sex: Unspecified
Mutation:
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-06-15
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 2
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: bioptonics
Image Quality: Low
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Neural tube, wing bu, hind limb bud
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2197
  Name: trunk lateral plate mesoderm
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2111
  Name: tail mesoderm
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
Back