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Assay:

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Id: 30244
Copied From:
 
GENERAL INFORMATION
 
Authors: Andrew Bain
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment: 30245
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: SEPT11_Probe_2_AS_DB
Gene Details
Gallus gallus Id: GG:15150
Entity Detected: SEPT11
Gene Name: septin 11 [Gallus gallus]
Entrez Id: 422635
Ensembl Id: ENSGALG00000011476
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed:
Stage: HH20
Stage Qualifier: Exact
Sex: Unspecified
Mutation:
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-07-18
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 3
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: bioptonics
Image Quality: Medium
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Neural tube, 1 st pharyngeal arch, 2nd Pharyngeal arch wing bud hind limb bud
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Weak
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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