| Id: |
30233 |
| Copied From: |
30232 |
| |
| GENERAL INFORMATION |
| |
| Authors: |
Andrew Bain |
| Lab: |
Roslin Institute |
| Standard Protocol Id: |
Roslin |
| Lab Book Reference: |
|
| Confidence: |
High |
| Status: |
Public |
| Notes For Interpretation: |
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| Linked Data - Replicated Experiment: |
30234,30235 |
| Linked Data - Same Experiment: |
|
| Linked Data - Same Embryo: |
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| |
| PROBE |
| |
| Probe: |
MCF2L_Probe_2_AS_DB |
| Gene Details |
| Gallus gallus Id: |
GG:2463 |
| Entity Detected: |
MCF2L |
| Gene Name: |
MCF.2 cell line derived transforming sequence-like [Gallus gallus] |
| Entrez Id: |
418748 |
| Ensembl Id: |
ENSGALG00000016834 |
| |
| Chemistry: |
RNA |
| Label: |
Digoxigenin |
| |
| SPECIMEN |
| |
| Organism: |
Gallus gallus |
| Breed: |
|
| Stage: |
HH20 |
| Stage Qualifier: |
Exact |
| Sex: |
Unspecified |
| Mutation: |
|
| Mounting: |
Wholemount |
| Fixative: |
4% PFA |
| Embedding Reagent: |
|
| |
| HYBRIDIZATION PROCEDURE |
| |
| Hybridization Date: |
2012-03-10 |
| Secondary: |
|
| Tertiary: |
|
| Quaternary: |
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| Final Label: |
Alkaline Phosphatase |
| Detected Using: |
NBT/BCIP concentration 2x lower than in Bath1 |
| Development Time: |
1 |
| Time Unit: |
Hours |
| |
| IMAGING PROCEDURE |
| |
| Image Technique: |
OPT |
| Clearing Agent: |
BABB |
| |
| IMAGE FILES |
| |
| Local Zip File or Comment: |
|
| Number of Image Sets: |
Pending |
| Image Folder: |
bioptonics |
| Image Quality: |
Medium |
| |
| EXPRESSION PATTERN DESCRIPTION |
| |
| Detected In: |
Neural tube, Trunk somites, otic vesicle ,neural tube,
tail mesoderm wing bud, hind limb bud |
| Not Detected In: |
|
| Possibly Detected In: |
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| |
| ANNOTATIONS |
| |
All site content,
except where otherwise noted,
