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Assay:

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Id: 30223
Copied From: 30222
 
GENERAL INFORMATION
 
Authors: Andrew Bain
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment: 30222,30224
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: RAB11FIP4-2_Probe_3_AS_DB
Gene Details
Gallus gallus Id: GG:7182
Entity Detected: RAB11FIP4-2
Gene Name: RAB11 family interacting protein 4 (class II) [Gallus gallus]
Entrez Id: 427808
Ensembl Id: ENSGALG00000003227
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH20
Stage Qualifier: Exact
Sex: Unspecified
Mutation:
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-07-28
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 3
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: photography
Image Quality: Medium
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: wing limb bud, Hind limb bud, tail somites, tail bud ,trunk somites, neural tube neural tube
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Weak
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1379
  Name: otic vesicle
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Weak
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2115
  Name: tail somite
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2106
  Name: tail bud
  Strength: Weak
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1026
  Name: lens vesicle
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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