| Id: |
30212 |
| Copied From: |
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| GENERAL INFORMATION |
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| Authors: |
Andrew Bain |
| Lab: |
Roslin Institute |
| Standard Protocol Id: |
Roslin |
| Lab Book Reference: |
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| Confidence: |
High |
| Status: |
Public |
| Notes For Interpretation: |
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| Linked Data - Replicated Experiment: |
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| Linked Data - Same Experiment: |
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| Linked Data - Same Embryo: |
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| PROBE |
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| Probe: |
PIK3CD_Probe_2_AS_DB |
| Gene Details |
| Gallus gallus Id: |
GG:10445 |
| Entity Detected: |
PIK3CD |
| Gene Name: |
phosphoinositide-3-kinase catalytic delta polypeptide [Gallus gallus] |
| Entrez Id: |
419444 |
| Ensembl Id: |
ENSGALG00000002583 |
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| Chemistry: |
RNA |
| Label: |
Digoxigenin |
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| SPECIMEN |
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| Organism: |
Gallus gallus |
| Breed: |
White Leghorn |
| Stage: |
HH19 |
| Stage Qualifier: |
Exact |
| Sex: |
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| Mutation: |
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| Mounting: |
Wholemount |
| Fixative: |
4% PFA |
| Embedding Reagent: |
Agarose |
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| HYBRIDIZATION PROCEDURE |
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| Hybridization Date: |
2011-08-01 |
| Secondary: |
Anti-Digoxigenin Ab |
| Tertiary: |
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| Quaternary: |
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| Final Label: |
Alkaline Phosphatase |
| Detected Using: |
NBT/BCIP concentration 2x lower than in Bath1 |
| Development Time: |
3 |
| Time Unit: |
Hours |
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| IMAGING PROCEDURE |
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| Image Technique: |
Photography |
| Clearing Agent: |
BABB |
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| IMAGE FILES |
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| Local Zip File or Comment: |
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| Number of Image Sets: |
Pending |
| Image Folder: |
photography |
| Image Quality: |
Medium |
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| EXPRESSION PATTERN DESCRIPTION |
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| Detected In: |
wing bud, hind limb bud, neural tube |
| Not Detected In: |
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| Possibly Detected In: |
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| ANNOTATIONS |
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All site content,
except where otherwise noted,
