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Assay:

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Id: 30206
Copied From: 30205
 
GENERAL INFORMATION
 
Authors: Andrew Bain
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 30205
Linked Data - Same Embryo:
 
PROBE
 
Probe: ChEST162f9_Probe_9_AS_DB
Gene Details
Gallus gallus Id: GG:0
Entity Detected: UNANNOTATED
Gene Name: Unknown
Entrez Id: Unknown
Ensembl Id: Unknown
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed:
Stage: HH19
Stage Qualifier: Exact
Sex: Unspecified
Mutation:
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-08-15
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 3
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: bioptonics
Image Quality: Medium
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: wing bud,hind limb bud,neural tube
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Weak
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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