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Assay:

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Id: 30198
Copied From: 30197
 
GENERAL INFORMATION
 
Authors: Andrew Bain
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment: 30197
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: SMOC1_Probe_3_AS_DB
Gene Details
Gallus gallus Id: GG:16520
Entity Detected: SMOC1
Gene Name: SPARC related modular calcium binding 1 [Gallus gallus]
Entrez Id: 768688
Ensembl Id: ENSGALG00000009415
 
Chemistry: RNA
Label:
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH21
Stage Qualifier: Exact
Sex: Unspecified
Mutation:
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2012-02-14
Secondary:
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT/BCIP concentration 2x lower than in Bath1
Development Time: 1
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: Pending
Image Folder: bioptonics
Image Quality: Medium
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: lens vesicle,trunk somites, tail somites, neural tube,lateral plate mesoderm. wing bud ,hind limb bud , tail bud ,pharyngeal arches:; I,II,III
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1026
  Name: lens vesicle
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2207
  Name: trunk somite
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2115
  Name: tail somite
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:974
  Name: lateral plate mesoderm
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2310
  Name: wing bud
  Strength: Weak
  Pattern: Regional
  Direction: Unknown
  Note: Anterior and posterior absent in AER
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2106
  Name: tail bud
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:4
  Name: 1st pharyngeal arch
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:23
  Name: 2nd branchial arch see 2nd pharyngeal arch)
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:41
  Name: 3rd pharyngeal arch
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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