| Id: |
30175 |
| Copied From: |
30174 |
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| GENERAL INFORMATION |
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| Authors: |
Andrew Bain |
| Lab: |
Roslin Institute |
| Standard Protocol Id: |
Roslin |
| Lab Book Reference: |
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| Confidence: |
High |
| Status: |
Public |
| Notes For Interpretation: |
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| Linked Data - Replicated Experiment: |
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| Linked Data - Same Experiment: |
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| Linked Data - Same Embryo: |
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| PROBE |
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| Probe: |
ChEST125i5_Probe_8_AS_DB |
| Gene Details |
| Gallus gallus Id: |
GG:0 |
| Entity Detected: |
UNANNOTATED |
| Gene Name: |
Unknown |
| Entrez Id: |
Unknown |
| Ensembl Id: |
Unknown |
| |
| Chemistry: |
RNA |
| Label: |
|
| |
| SPECIMEN |
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| Organism: |
Gallus gallus |
| Breed: |
White Leghorn |
| Stage: |
HH17 |
| Stage Qualifier: |
Exact |
| Sex: |
Unspecified |
| Mutation: |
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| Mounting: |
Wholemount |
| Fixative: |
4% PFA |
| Embedding Reagent: |
Agarose |
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| HYBRIDIZATION PROCEDURE |
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| Hybridization Date: |
2011-08-20 |
| Secondary: |
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| Tertiary: |
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| Quaternary: |
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| Final Label: |
Alkaline Phosphatase |
| Detected Using: |
NBT/BCIP concentration 2x lower than in Bath1 |
| Development Time: |
15 |
| Time Unit: |
Minutes |
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| IMAGING PROCEDURE |
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| Image Technique: |
OPT |
| Clearing Agent: |
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| IMAGE FILES |
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| Local Zip File or Comment: |
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| Number of Image Sets: |
Pending |
| Image Folder: |
bioptonics |
| Image Quality: |
Medium |
| |
| EXPRESSION PATTERN DESCRIPTION |
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| Detected In: |
Somites,neural tube,wing bud,hind limb bud,somitic mesoderm,myotome |
| Not Detected In: |
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| Possibly Detected In: |
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| ANNOTATIONS |
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All site content,
except where otherwise noted,
