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Assay:

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Id: 30171
Copied From: 30170
 
GENERAL INFORMATION
 
Authors: Andrew Bain
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment: 30172, 30173
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: SMOC1_Probe_3_AS_DB
Gene Details
Gallus gallus Id: GG:16520
Entity Detected: SMOC1
Gene Name: SPARC related modular calcium binding 1 [Gallus gallus]
Entrez Id: 768688
Ensembl Id: ENSGALG00000009415
 
Chemistry: RNA
Label:
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH20
Stage Qualifier: Exact
Sex: Unspecified
Mutation:
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2012-07-27
Secondary:
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 2
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Wing bud, neural tube, tail bud, somites,, hind limb bud, lens vesicle, tail somitic mesoderm
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1026
  Name: lens vesicle
  Strength: Weak
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1281
  Name: neural tube
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2111
  Name: tail mesoderm
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:20
  Name: 2nd hyoid pharyngeal arch
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:41
  Name: 3rd pharyngeal arch
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2207
  Name: trunk somite
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:2115
  Name: tail somite
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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