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Assay:

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Id: 40955
Copied From: 40570
 
GENERAL INFORMATION
 
Authors: Pang Sze Wing Angel
Lab: University College London
Standard Protocol Id: UCL OPT
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 40956| 40957
Linked Data - Same Embryo:
 
PROBE
 
Probe: MSX2_Probe_3_AS_CS
Gene Details
Gallus gallus Id: GG:5040
Entity Detected: MSX2
Gene Name: msh homeobox 2 [Gallus gallus]
Entrez Id: 395245
Ensembl Id: ENSGALG00000002947
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH21
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-07-07
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 9
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References:
 
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