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Assay:

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Id: 40903
Copied From: 40885
 
GENERAL INFORMATION
 
Authors: Jackie Liu
Lab: University College London
Standard Protocol Id: ucl
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 40885
Linked Data - Same Embryo: 40885
 
PROBE
 
Probe: GNPDA1_Probe_2_AS_DB
Gene Details
Gallus gallus Id: GG:5307
Entity Detected: GNPDA1
Gene Name: glucosamine-6-phosphate deaminase 1 [Gallus gallus]
Entrez Id: 416341
Ensembl Id: ENSGALG00000007312
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Rhode Island Red
Stage: HH14
Stage Qualifier: Late (+)
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2012-08-02
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 12
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1282
  Name: neural tube floor plate
  Strength: Weak
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:142
  Name: anterior intestinal portal
  Strength: Weak
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:2106
  Name: tail bud
  Strength: Moderate
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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