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Assay:

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Id: 20119
Copied From: 20118
 
GENERAL INFORMATION
 
Authors: Gordana / Monique
Lab: University of Bath
Standard Protocol Id: Bath1
Lab Book Reference: 05-06-2009
Confidence: Low
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20017|20018
Linked Data - Same Embryo:
 
PROBE
 
Probe: CCND2-1_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:1403
Entity Detected: CCND2-1
Gene Name: cyclin D2 [Gallus gallus]
Entrez Id: 374047
Ensembl Id: ENSGALG00000017283
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH27
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-06-05
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 34
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: limb mesenchyme|first pharyngeal arch: maxillary process|first pharyngeal arch: mandibular process|neural tube
Not Detected In:
Possibly Detected In: spinal cord neurons
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References:
 
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