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Assay:

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Id: 30120
Copied From:
 
GENERAL INFORMATION
 
Authors: Frances Wong
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: Low
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 30119, 30121
Linked Data - Same Embryo:
 
PROBE
 
Probe: GSC_Probe_1_AS_DB
Gene Details
Gallus gallus Id: GG:16817
Entity Detected: GSC
Gene Name: goosecoid homeobox [Gallus gallus]
Entrez Id: 396273
Ensembl Id: ENSGALG00000010974
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH18
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent: Agarose
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-08-03
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1470
  Name: pharyngeal arch
  Strength: Possible
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:228
  Name: brain
  Strength: Possible
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:746
  Name: hind limb
  Strength: Possible
  Pattern: Regional
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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