| Id: |
30111 |
| Copied From: |
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| GENERAL INFORMATION |
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| Authors: |
Megan Davey |
| Lab: |
Roslin Institute |
| Standard Protocol Id: |
Roslin |
| Lab Book Reference: |
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| Confidence: |
High |
| Status: |
Public |
| Notes For Interpretation: |
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| Linked Data - Replicated Experiment: |
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| Linked Data - Same Experiment: |
30112 |
| Linked Data - Same Embryo: |
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| PROBE |
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| Probe: |
IFT20_Probe_1_AS_DB |
| Gene Details |
| Gallus gallus Id: |
GG:7778 |
| Entity Detected: |
IFT20 |
| Gene Name: |
intraflagellar transport 20 homolog (Chlamydomonas) [Gallus gallus] |
| Entrez Id: |
417671 |
| Ensembl Id: |
ENSGALG00000005707 |
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| Chemistry: |
RNA |
| Label: |
Digoxigenin |
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| SPECIMEN |
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| Organism: |
Gallus gallus |
| Breed: |
ISA Brown |
| Stage: |
HH23 |
| Stage Qualifier: |
Exact |
| Sex: |
Unspecified |
| Mutation: |
None (Wild-Type) |
| Mounting: |
Wholemount |
| Fixative: |
4% PFA |
| Embedding Reagent: |
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| HYBRIDIZATION PROCEDURE |
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| Hybridization Date: |
2011-03-10 |
| Secondary: |
Anti-Digoxigenin Ab |
| Tertiary: |
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| Quaternary: |
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| Final Label: |
Alkaline Phosphatase |
| Detected Using: |
NBT+BCIP |
| Development Time: |
30 |
| Time Unit: |
Minutes |
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| IMAGING PROCEDURE |
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| Image Technique: |
Photography |
| Clearing Agent: |
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| IMAGE FILES |
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| Local Zip File or Comment: |
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| Number of Image Sets: |
1 |
| Image Folder: |
photography |
| Image Quality: |
High |
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| EXPRESSION PATTERN DESCRIPTION |
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| Detected In: |
Branchial arches I, II, III and IV, head mesenchyme, somites and limbs. |
| Not Detected In: |
Central region of the limbs. |
| Possibly Detected In: |
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| ANNOTATIONS |
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All site content,
except where otherwise noted,
